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http://www.repositorio.cdtn.br:8080/jspui/handle/123456789/527| Title: | Biochemical characterization of neutral trehalase activity in saccharomyces boulardii |
| Title of periodic: | World Journal of Microbiology and Biotechnology |
| Authors: | Andrade, Antero Silva Ribeiro de Santos, Raquel Gouvea dos Nicoli, Jacques R. Neves, Maria José |
| Affiliation: | Centro de Desenvolvimento da Tecnologia Nuclear/CDTN, Belo Horizonte, MG, Brasil Centro de Desenvolvimento da Tecnologia Nuclear/CDTN, Belo Horizonte, MG, Brasil Universidade Federal de Minas Gerais/UFMG, Belo Horizonte, MG, Brasil Centro de Desenvolvimento da Tecnologia Nuclear/CDTN, Belo Horizonte, MG, Brasil |
| Issue Date: | 2000 |
| Keywords: | Saccharomyces boulardii;Trehalases;yeasts |
| Abstract: | The aim of the present study was to characterize trehalase activity in this yeast. Cells of S. boulardii grown in glucose exhibited neutral trehalase activity only in the exponential phase. Acidic trehalase was not detected in glucose medium. Cells grown in trehalose exhibited acid and neutral trehalase activities at all growth stages, particularly in the exponential phase. The optimum pH and temperature values for neutral trehalase activity were determined as 6.5 and 30 Celsius degree respectively, the half-life being approximately 3 min at 45 Celsius degree. |
| Access: | L |
| Appears in Collections: | Artigo de periódico |
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